The Cytokine Human 10-Plex Panel for the Luminex? platform is specifically designed for quantifying human GM-CSF, IFN-γ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10 and TNF-α in serum, plasma, and tissue culture supernatant. By measuring 10 analytes simultaneously, the Luminex? assay panel helps provide more data from each sample, saving both money and time compared to more traditional systems (such as ELISA) used in research. This assay may be run alone or in combination with other select Luminex? singleplex kits. The panel is suitable for use with the Luminex? 100?/200? and FLEXMAP 3D? systems.
??Superior performance—accurate, reproducible, and sensitive quantitation of multiple proteins ??High quality—fully qualified antibodies permit excellent specificity and sensitivity ??Fast and easy protocols—perform your multiplex assay and analyze your data typically in less than one day
Our Comprehensive Human Analyte Panel The Human Cytokine 10-Plex Panel for the Luminex? platform provides reagents for the accurate, reproducible, and sensitive quantitation of human proteins including:
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Cytokines
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GM-CSF
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IFN-γ
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IL-1β
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IL-2
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IL-4
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IL-5
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IL-6
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IL-8
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IL-10
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TNF-α
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Luminex? xMAP? Technology—Effective and Proven Analytical Tool The Human Cytokine 10-Plex Panel is based on xMAP? technology. The use of a suspension bead-based technology enables the multiplexing capabilities of the Luminex? assays. Polystyrene microspheres are internally dyed with red and infrared fluorophores of differing intensities. Each bead is given a unique number, or bead region, allowing differentiation of one bead from another. Beads covalently bound to different antibodies can be mixed in the same assay, utilizing a 96-well microplate format. Upon completion of the sandwich immunoassay, beads must be measured using a?Luminex? detection system(100?/200? or FLEXMAP 3D?). The instrument uses xPONENT? software to distinguish bead color (analyte) and R-PE fluorescence intensity (assay signal strength) to quantify target(s).
More Validation for Confidence in Your Results The Human Cytokine 10-Plex Panel has been validated using the same rigorous criterion that distinguishes Luminex? assays from other commercially available assays. With more than 20 years of experience, our fully qualified antibodies help ensure the specificity and sensitivity of our assays. When benchmarked against the competition, our multiplex products consistently deliver excellent results. Every singleplex kit and premixed multiplex kit comes with a product insert that outlines the specifications for each marker in the assay (see Documents section below). Standard curves (Figure 1 and 2) are generated for each lot of product to assure quantitation consistency.
